Figure 8.

Effect of WKS1.1 on tAPX Activity in Vitro and in Planta.

(A) tAPX-specific activity in the presence of GST+ATP (negative control), KA+ATP, and KA without ATP (n = 4, *P < 0.05; KA = kinase alone). Error bars are standard errors of the means.

(B) APX-specific activity in thylakoid fractions from Bobwhite plants transformed with NP:GFP (control) and with NP:WKS1 (genomic). Dunnett’s tests, *P < 0.05 and **P < 0.01

(C) APX-specific activity in soluble fractions extracted from the same genotypes as in (B). No significant differences were detected.

(B) and (C) 26b15, 26b6, and 17a15 are three independent transformation events with a genomic copy of WKS1 (Fu et al., 2009). Error bars are standard errors of the means.

(D) F3 families segregating for ΔtAPX-6B deletion and Ubi:TAP-WKS1.1 transgene infected with Pst race PST130. Plants carrying WKS1.1 showed partial resistance and plants without the transgene were susceptible. The ΔtAPX-6B deletion did not affect Pst resistance.