Skip to main content
. 2015 May 19;27(6):1755–1770. doi: 10.1105/tpc.114.134296

Table 1. Transgenic Wheat Plants Used in This Study.

Construct Target Gene Vector Backbone Promoter Transformation Method Bact. Selectiona Plant Selection Host Variety Purpose
NP:WKS1.1 WKS1.1 pRLF10 Native WKS1b Agro amp 100, kan 20 G418 T. aestivum Glasgow Function of transcript variant
NP:WKS1.2 WKS1.2 pRLF10 Native WKS1b Agro amp 100, kan 20 G418 T. aestivum Glasgow Function of transcript variant
Ubi:TAP-WKS1.1 WKS1.1 pCAMBia1300c Maize Ubi Bombardment kan 50 Bialaphos T. aestivum Bobwhite CoIP
NP:WKS1.1-GFP WKS1.1 pGWB4d Native WKS1b Bombardment kan 50 Bialaphos T. aestivum Bobwhite Subcellular localization
NP:GFP GFP pGWB4d Native WKS1b Bombardment kan 50 Bialaphos T. aestivum Bobwhite Subcellular localization control
a

amp, ampicillin; kan, kanamycin.

b

Native WKS1 promoter includes 3543 bp upstream from starting codon.