Table 1. Transgenic Wheat Plants Used in This Study.
Construct | Target Gene | Vector Backbone | Promoter | Transformation Method | Bact. Selectiona | Plant Selection | Host Variety | Purpose |
---|---|---|---|---|---|---|---|---|
NP:WKS1.1 | WKS1.1 | pRLF10 | Native WKS1b | Agro | amp 100, kan 20 | G418 | T. aestivum Glasgow | Function of transcript variant |
NP:WKS1.2 | WKS1.2 | pRLF10 | Native WKS1b | Agro | amp 100, kan 20 | G418 | T. aestivum Glasgow | Function of transcript variant |
Ubi:TAP-WKS1.1 | WKS1.1 | pCAMBia1300c | Maize Ubi | Bombardment | kan 50 | Bialaphos | T. aestivum Bobwhite | CoIP |
NP:WKS1.1-GFP | WKS1.1 | pGWB4d | Native WKS1b | Bombardment | kan 50 | Bialaphos | T. aestivum Bobwhite | Subcellular localization |
NP:GFP | GFP | pGWB4d | Native WKS1b | Bombardment | kan 50 | Bialaphos | T. aestivum Bobwhite | Subcellular localization control |
amp, ampicillin; kan, kanamycin.
Native WKS1 promoter includes 3543 bp upstream from starting codon.