Figure 2.

Zebularine Effects on DNA Methylation and Nucleoprotein Adduct Formation.

(A) Percentage of DNA methylation in dissected shoot apices based on bisulfite sequencing of 24 h and 5 d mock- and 20 μM zebularine (zeb)-treated samples. A minimum number of 12 reads per experimental point has been analyzed. Schematic view of the analyzed genomic regions is provided in Supplemental Figure 1.

(B) RT-qPCR measurement of DNA damage marker gene induction in the wild type (WT) and drm1 drm2 cmt3 (ddc) triple mutant after 24 h treatment with mock and 20 μM zebularine normalized to ACTIN7. Error bars represent sd of three biological replicates and asterisks P < 0.05 in t test.

(C) Relative root length of wild-type and ddc plants in response to 20 μM zebularine, 15 μM MMC, or 50 nM bleocin treatment. Error bars represent sd of three biological replicates and asterisk P < 0.05 in t test.