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. 2015 Jun 12;27(6):1634–1649. doi: 10.1105/tpc.15.00110

Figure 1.

Figure 1.

JA Induces Leaf Senescence in a COI1-JAZ-Dependent Manner.

(A) The senescence phenotype of leaves detached from 3-week-old Arabidopsis Col-0 (WT), coi1-1, and JAZ1Δ3A and treated without (Mock) or with 100 μM MeJA in the dark for 6 d.

(B) to (D) Chlorophyll content (B), photochemical efficiency (Fv/Fm) of PSII (C), and membrane ion leakage (D) in the detached leaves with the same treatment as shown in (A). The maximum value of the chlorophyll contents in (B) is 2.09 mg/g fresh weight (FW). Data are means (±sd) of three biological replicates. Asterisks represent Student’s t test significance between wild-type and coi1-1 or JAZ1Δ3A samples (**P < 0.01).

(E) Quantitative real-time PCR analysis for senescence-associated genes (SAG12, SAG13, SAG29, SAG113, and SEN4) and photosynthetic genes (CAB1 and RBCS) of the detached leaves with the same treatment as shown in (A). The y axis is the relative mRNA abundance of the detected genes. The mRNA abundance of the detected gene in mock-treated wild type with ACTIN8 as the internal control was set to 1. Data are means (±sd) of three biological replicates. Asterisks represent Student’s t test significance between wild-type and coi1-1 or JAZ1Δ3A samples (**P < 0.01).