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. 2015 Jun 12;27(6):1634–1649. doi: 10.1105/tpc.15.00110

Figure 5.

Figure 5.

Loss of Function of the IIId bHLH Factors Accelerates JA-Induced Leaf Senescence.

(A) The senescence phenotype of leaves that were detached from 3-week-old Arabidopsis Col-0 (WT) and the bhlh03 bhlh13 bhlh14 bhlh17 (bhlh03/13/14/17) quadruple mutant of the IIId bHLH factors (bHLH03, bHLH13, bHLH14, and bHLH17) and treated without (Mock) or with 100 μM MeJA in the dark for 5 d.

(B) to (E) Chlorophyll content (B), photochemical efficiency (Fv/Fm) of PSII (C), membrane ion leakage (D), and quantitative real-time PCR analysis for senescence-associated genes (SAG12, SAG13, SAG29, SAG113, and SEN4) and photosynthetic genes (CAB1 and RBCS) (E) of the detached leaves with the same treatment as shown in (A). The maximum value of the chlorophyll contents in (B) is 2.10 mg/g FW. The y axis in (E) is the relative mRNA abundance of the detected genes, and the mRNA abundance of the detected gene in mock-treated wild type with ACTIN8 as the internal control was set to 1. Data are means (±sd) of three biological replicates. Asterisks represent Student’s t test significance between wild-type and bhlh03 bhlh13 bhlh14 bhlh17 samples (**P < 0.01).