Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 22;27(6):1620–1633. doi: 10.1105/tpc.15.00116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 American Society of Plant Biologists. All rights reserved.

PMC Copyright notice

Figure 6. — MYC2, MYC3, MYC4, and MYC5 Interact with MYB21 and MYB24.

(A) Schematic diagrams of MYB21, MYB24, and their N-terminal domains (MYB21NT and MYB24NT). MYB21NT and MYB24NT contain the conserved R2 (red) and R3 (blue) domains but not the NYW^G/_S^M/_VDD^I/_LW^S/_P motif (orange).

(B) Y2H assay to show interactions between MYB21NT/MYB24NT and MYC5. MYB21NT and MYB24NT were fused with the DNA binding domain (BD), and MYC5 was fused with the activation domain (AD).

(C) Co-IP assay to verify the interaction between MYC5 and MYB21 in vivo. Flag-MYC5 was coexpressed without (empty control) or with myc-MYB21 or myc-COI1 (negative control) in N. benthamiana leaves. The total protein extracts from N. benthamiana leaves transiently expressing flag-MYC5, flag-MYC5 plus myc-MYB21, or flag-MYC5 plus myc-COI1 were immunoprecipitated using the anti-c-myc antibody-conjugated agarose and were blotted with anti-flag or anti-c-myc antibody.

(D) BiFC assay to detect interactions of MYC2, MYC3, MYC4, and MYC5 with MYB21 and MYB24. MYC2, MYC3, MYC4, and MYC5 were fused with nYFP, and MYB21 and MYB24 were fused with cYFP. The nuclei are indicated by 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining.