Figure 7.

Interaction of KAT1 in Vivo Depends on VAMP721 Residue Tyr-57.

(A) rBiFC analysis of YFP and RFP fluorescence collected from tobacco transformed using the pBiFCt-2in1-NC 2in1 vector (Figure 2). Images are (left to right) YFP (BiFC) fluorescence, RFP fluorescence, and bright field. Constructs (top to bottom) included coding sequences for KAT1-cYFP alone and with nYFP-X fusions of iLOV, VAMP721^Y57A, VAMP721^Y57D, VAMP721^D61A, VAMP721^Q76A, VAMP721^S80A, and VAMP723^D57Y. KAT1 and VAMP constructs were detected by αHA and αmyc antibodies to verify fusion protein expression (right). Bar = 10 μm.

(B) rBiFC fluorescence signals from three independent experiments. Each bar represents the mean ± se of fluorescence intensity ratios from 10 images taken at random over the leaf surface. rBiFC signals were calculated as the mean fluorescence intensity ratio determined from each image set after subtracting the background fluorescence determined from an equivalent number of images taken from nontransformed tissues. Significance is indicated by letters at P < 0.01.