. 2015 May 22;27(6):1697–1717. doi: 10.1105/tpc.15.00305

Table 3. Suppressing KC1-AKT1 K⁺ Current in X. laevis Oocytes by VAMP721 Depends on the Longin Domain Residue Tyr-57.

	V_1/2 (mV)	g_max (ms)	δ
AKT1	−93.1 ± 1.0	0.52 ± 0.01	−1.45 ± 0.03
AKT1+KC1	−186.8 ± 2.1
AKT1+KC1+VAMP721	−212.2 ± 3.2*
AKT1+KC1+VAMP723	−189.2 ± 1.4
AKT1+KC1+VAMP721^Y57A	−209.7 ± 3.1*
AKT1+KC1+VAMP721^Y57D	−187.1 ± 2.4

Parameter values are results of joint, nonlinear least-squares fitting of K⁺ currents in Figure 10. Fittings were carried out with the gating charge, δ, and g_max held in common, and values for the voltage giving half-maximal conductance V_1/2 were allowed to vary between data sets. Data are from seven or more separate experiments for each construct combination and are given as means ± se. Significance for V_1/2 as the difference from KC1-AKT1 expressed alone at *P < 0.01.

Table 3. Suppressing KC1-AKT1 K+ Current in X. laevis Oocytes by VAMP721 Depends on the Longin Domain Residue Tyr-57.

Table 3. Suppressing KC1-AKT1 K⁺ Current in X. laevis Oocytes by VAMP721 Depends on the Longin Domain Residue Tyr-57.