Figure 2. Effect of in vivo chronic leptin administration on aquaglyceroporins AQP3 and AQP7 expression and tissue distribution in adipose tissue of wild type and ob/ob mice.

Immunohistochemical detection of AQP3 (A) and AQP7 (B) in subcutaneous white adipose tissue (WAT) of wild type (left panels) and ob/ob (right panels) mice (magnification 200X, scale bar = 50 μm). Bar graphs show transcript and protein levels of AQP3 (C, E) and AQP7 (D, F) in subcutaneous WAT obtained from vehicle-treated, pair-fed and leptin-treated wild type and ob/ob mice. The gene and protein expression in vehicle-treated wild type mice was assumed to be 1. Representative blots are shown at the bottom of the figure. Differences between groups were analyzed by two-way ANOVA.