Figure 4. Positive association of Pparg transcript levels with aquaglyceroporins Aqp7 and Aqp9 in adipose tissue and liver of experimental animals.

Bar graphs illustrate the changes in Pparg mRNA levels in subcutaneous white adipose tissue (WAT) (A) and liver (B) obtained from vehicle-treated, pair-fed and leptin-treated wild type and ob/ob mice. A positive correlation was found between Pparg and Aqp7 mRNA in subcutaneous WAT (C) as well as between Pparg and Aqp9 transcript levels in liver (D) of experimental groups. The Pearson’s correlation coefficient (r) and P values are indicated. Effect of leptin stimulation for 24 h on basal and thiazolidinedione (TZD) rosiglitazone (10 μmol/L)-induced expression of Pparg (E, F) and aquaglyceroporins Aqp7 and Aqp9 (G, H) in murine differentiated subcutaneous adipocytes and AML12 hepatocytes. The gene expression in vehicle-treated wild type mice or unstimulated cells was assumed to be 1. Differences between groups were analyzed by two-way ANOVA or one-way ANOVA followed by Tukey’s post-hoc test, where appropriate. *P < 0.05; **P < 0.01 vs. control unstimulated cells.