Table 1. Markers of adiposity, body temperature and lipolysis of experimental groups.
| Wild type |
ob/ob |
|||||
|---|---|---|---|---|---|---|
| Control | Pair-fed | Leptin | Control | Pair-fed | Leptin | |
| n | 9 | 10 | 9 | 10 | 10 | 9 |
| Body weight (g)a,b,c | 25.4 ± 0.2 | 22.6 ± 0.1* | 22.6 ± 0.1* | 49.2 ± 0.8* | 33.6 ± 0.4*,† | 24.9 ± 0.2†,$ |
| Cumulative food intake (g)a,b,c | 94 ± 2 | 88 ± 1 | 88 ± 1 | 191 ± 5* | 66 ± 5*,† | 66 ± 5*,† |
| Rectal temperature (°C)c | 35.4 ± 0.2 | 36.0 ± 0.2 | 35.4 ± 0.1 | 35.7 ± 0.2 | 34.7 ± 0.3† | 36.0 ± 0.2$ |
| Epididymal WAT (g)a,b,c | 0.19 ± 0.01 | 0.13 ± 0.02 | 0.04 ± 0.01 | 1.64 ± 0.10* | 1.04 ± 0.05*,† | 0.46 ± 0.06*,†,$ |
| Subcutaneous WAT (g)a,b,c | 0.17 ± 0.01 | 0.15 ± 0.02 | 0.09 ± 0.05 | 3.07 ± 0.32* | 1.85 ± 0.11*,† | 0.47 ± 0.06*,†,$ |
| Perirrenal WAT (g)a,b,c | 0.05 ± 0.01 | 0.03 ± 0.01 | 0.01 ± 0.01 | 0.92 ± 0.05* | 0.51 ± 0.04*,† | 0.09 ± 0.01*,†,$ |
| Total white adiposity (g)a,b,c | 0.42 ± 0.04 | 0.31 ± 0.01 | 0.14 ± 0.05 | 5.62 ± 0.38* | 3.40 ± 0.23*,† | 1.03 ± 0.13*,†,$ |
| FFA (mg/dL)b | 44 ± 8 | 45 ± 5 | 32 ± 4 | 39 ± 5 | 55 ± 11 | 24 ± 2 |
| Glycerol (mmol/mL)a,b,c | 0.05 ± 0.01 | 0.03 ± 0.01 | 0.02 ± 0.01* | 0.07 ± 0.01* | 0.05 ± 0.01 | 0.01 ± 0.01*,†,$ |
FFA, free fatty acids; WAT, white adipose tissue. Values presented as the mean ± SEM. Differences between groups were analyzed by two-way ANOVA or one-way ANOVA followed by Tukey’s post-hoc test when an interaction between factors was detected. aP < 0.05, effect of genotype; bP < 0.05 effect of treatment; cP < 0.05 interaction between genotype and treatment. *P < 0.05 vs. vehicle-treated wild type mice; †P < 0.05 vs. vehicle-treated ob/ob mice; #P < 0.05 vs. pair-fed wild type mice; $P < 0.05 vs. pair-fed ob/ob mice.