Figure 3.
ECM protein coating of culture plates influences EaHy-926 endothelial cells adherence and spreading.After 4 hours in culture EaHy-926 endothelial cells were observably more spread on culture plates coated with fibronectin than on plates coated with type I collagen or decorin. Representative phase contrast images are shown of EaHy-926 endothelial 4 hours post seeding. B: After 4 hours in culture the average cell area was significantly greater for EaHy-926 endothelial cells on fibronectin compared to those cells on either type I collagen or decorin. Data shown are means ± SEM in relative units (**=p<0.01, ***=p<0.001, Mann Whitney U test).