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. 2015 May 30;11(1):18–24. doi: 10.46582/jsrm.1101004

Figure 4.

Figure 4

EaHy-926 endothelial cells closed scratch wounds faster in MSC-CM than in unconditioned medium on type I collagen, fibronectin, and decorin coated culture plates. A: EaHy-926 cells on fibronectin coated culture plates closed scratch wounds significantly faster than those on either type I collagen or decorin. Representative phase contrast images are shown of EaHy-926 scratch assays 12 hours post scratching. B: After 12 hours EaHy-926 endothelial cells in MSC-CM had closed scratch wounds by a significantly greater degree than those in unconditioned control medium. 12 hours post-scratching, EaHy-926 endothelial cells on plates coated fibronectin had closed scratch wounds by a significantly greater degree than those on either type I collagen or decorin. On fibronectin and decorin, medium conditioned by MSC-2 was associated with significantly reduced scratch closure compared to either MSC-1 or MSC-3. On type one collagen, medium conditioned by MSC-2 was associated with significantly reduced scratch closure compared to MSC-1. Data shown are means ± SEM. (* = p<0.05 by Mann Whitney U test).