Table 1. Differential proteins between healthy and viruliferous Rhopalosiphum padi as identified by iTRAQ.

			12 h AAPc : 0 h AAP		24 h AAP : 0 h AAP		48 h AAP : 0 h AAP
Protein and pathway	%Cov (95)a	Peptides (95%)b	Ratiod	Pvale	Ratio	PVal	Ratio	PVal	Accession
Metabolic pathways
PREDICTED: glycerol-3-phosphate dehydrogenase, mitochondrial-like isoform 4	14.58	16	4.09	0.0204	0.92	0.7964	0.43	0.2628	gi|328709677
PREDICTED: glutamate dehydrogenase, mitochondrial-like	36.31	30	3.91	0.0004	2.11	0.0162	2.25	0.0503	gi|193582510
ATP synthase subunit beta, mitochondrial	55.47	75	3.22	0.0204	3.56	0.0155	0.76	0.5102	gi|209915626
putative mitochondrial ATP synthase alpha subunit precursor	49.00	63	3.56	0.0136	3.22	0.0204	0.96	0.3627	gi|52630965
PREDICTED: probable aconitate hydratase, mitochondrial-like	21.17	23	2.99	0.0195	1.11	0.3415	0.29	0.4397	gi|328716624
PREDICTED: acetyl-CoA carboxylase-like isoform 4	24.69	74	2.03	0.0075	2.11	0.0035	1.53	0.0795	gi|328714419
PREDICTED: glyceraldehyde-3-phosphate dehydrogenase-like	42.17	32	0.25	0.0353	1.13	0.3332	0.17	0.0047	gi|193688110
PREDICTED: glucosamine-fructose-6-phosphate aminotransferase [isomerizing] 2-like isoform 1	8.09	7	0.21	0.0464	0.58	0.1816	0.36	0.0977	gi|328718712
PREDICTED: elongation factor 2-like	31.64	49	0.16	0.0001	0.94	0.3257	0.33	0.0180	gi|193690671
ATP citrate lyase	41.21	60	1.28	0.7255	3.16	0.0238	2.19	0.1524	gi|237874159
peroxiredoxin-6-like	49.09	18	2.25	0.0613	2.29	0.0436	2.01	0.0231	gi|240848687
PREDICTED: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial-like	13.28	7	0.94	0.8571	0.44	0.1259	0.06	0.0067	gi|193587168
Ribosome
PREDICTED: 40 S ribosomal protein S8-like	46.15	21	0.23	0.0114	0.92	0.9471	0.69	0.5592	gi|328709805
40 S ribosomal protein S2-like	40.38	21	0.08	0.0397	1.24	0.8129	1.45	0.9157	gi|240849555
ribosomal protein S13e-like	29.14	5	0.17	0.0229	0.70	0.3303	1.29	0.7132	gi|242247489
uncharacterized protein LOC100165190	2.79	1	0.90	0.8412	1.04	0.9312	0.01	0.0453	gi|350535082
ACYPI010200	31.98	17	0.21	0.0281	0.67	0.7261	0.44	0.4620	gi|239789423
Regulation of actin cytoskeleton
PREDICTED: vinculin-like	18.15	17	1.42	0.0361	1.67	0.0350	1.61	0.0497	gi|193606151
PREDICTED: myosin heavy chain, muscle isoform 1	49.72	233	9.64	4.43E-05	5.40	1.06E-05	1.75	0.1814	gi|328702403
Signaling pathway
PREDICTED: voltage-dependent anion-selective channel-like isoform 1	25.27	10	0.95	0.3256	1.32	0.3841	0.17	0.0160	gi|193690508
putative activated protein kinase C receptor	54.86	24	0.54	0.0629	0.58	0.0984	0.45	0.0496	gi|52630921
Protein processing
PREDICTED: heat shock protein 83-like	44.37	63	0.31	0.0867	0.58	0.0554	0.28	0.0450	gi|193652748
PREDICTED: translocon-associated protein subunit gamma-like	5.95	2	1.07	0.8883	1.14	0.7876	0.01	0.0498	gi|328698093
Synaptic vesicle cycle
vesicle-associated membrane protein-like	31.43	4	0.01	0.0478	0.86	0.7880	0.38	0.2442	gi|240848629
RNA degradation
symbionin symL-pea aphid	39.05	56	0.28	0.0021	0.47	0.1640	0.13	0.0173	gi|285430
Unknown
myofilin isoform a	42.59	16	5.30	0.0365	2.78	0.2643	2.56	0.1109	gi|253735723
PREDICTED: paramyosin, long form-like	44.71	81	2.54	0.0027	1.89	0.0377	0.63	0.9907	gi|328724595
PREDICTED: staphylococcal nuclease domain-containing protein 1-like	34.02	49	0.43	0.0047	0.65	0.2712	0.43	0.0128	gi|193688302
cuticular protein 62 precursor	32.87	9	2.13	0.1359	0.44	0.0262	0.60	0.1887	gi|288558725
PREDICTED: KH domain-containing, RNA-binding, signal transduction-associated protein 3-like	20.23	10	0.77	0.0560	0.85	0.6793	0.63	0.0059	gi|193688146
putative histone h4-like protein, partial	52.48	23	1.20	0.4071	0.96	0.8159	0.62	0.0376	gi|604788259
glutathione S-transferase delta 1	26.56	6	0.51	0.1068	1.64	0.3014	0.25	0.0351	gi|392584108
PREDICTED: hypothetical protein LOC100164538	46.15	67	0.24	0.1818	0.74	0.6521	0.02	0.0481	gi|193706873

^aPercentage of matching amino acids from identified peptides having confidence ≥95%.

^bNumber of distinct wpeptides having at least 95% confidence.

^cAcquisition access period (AAP) on BYDV-GPV-infected wheat plants.

^dFold-change between two samples, ratio >1 represented up-regulation, ratio <1 represented down-regulation.

^fEvaluation of significance of fold-change.