Table 2. Putative proteins of Rhopalosiphum padi that interacted with the CP or RTP of Barley yellow dwarf virus-GPV in a yeast two-hybrid assay.
| Protein description and pathway | e-Value | Identity | Accession |
|---|---|---|---|
| Metabolic pathways | |||
| V-type proton ATPase subunit db | 2.00E-34 | 98% | NP_001191854.1 |
| Pancreatic lipase-related proteinab | 9.70E-02 | 32% | EGI64115.1 |
| Ubiquitin carboxyl-terminal hydrolaseb | 1.00E-105 | 81% | XP_008186680.1 |
| Ribosome | |||
| ribosomal protein L30-like proteinb | 3.00E-07 | 100% | NP_001119630.1 |
| 60 S ribosomal protein L8-likeb | 2.4 | 100% | NP_001155749.1 |
| Regulation of actin cytoskeleton | |||
| Twinstarb | 1.00E-46 | 99% | NP_001119642.1 |
| Signaling pathway | |||
| guanine nucleotide-binding protein G(s) subunit alphab | 0.032 | 100% | XP_001944148.1 |
| Protein processing | |||
| t-complex protein 1 subunit theta-likeb | 0.32 | 100% | XP_001944638.1 |
| Synaptic vesicle cycle | |||
| complexinb | 1.00E-25 | 99% | NP_001156645.1 |
| Neuroactive ligand-receptor interaction | |||
| neuronal acetylcholine receptor subunit alpha-7-likeb | 2.00E-35 | 97% | XP_001945224.2 |
| glutamate receptor ionotropic kainate 3-like isoform X3b | 3.00E-56 | 98% | XP_003242706.1 |
| Proteasome | |||
| proteasome beta 3 subunit-likeb | 3.00E-147 | 98% | NP_001155482.1 |
| Cardiac muscle contraction | |||
| tropomyosinb | 6.00E-91 | 85% | XP_008178569.1 |
| Ribosome biogenesis in eukaryotes | |||
| H/ACA ribonucleoproteina | 1.00E-24 | 96% | XP_003248969.1 |
| Unknown | |||
| cytochrome P450 4g15b | 4.00E-31 | 94% | XP_001944205.2 |
| cytochrome c oxidase polypeptide IV-likeab | 5.00E-109 | 93% | NP_001156047.1 |
| nascent polypeptide-associated complex subunit alphaab | 1.00E-94 | 99% | NP_001156301.1 |
| cytochrome c oxidase subunit Va-likeb | 3.00E-97 | 89% | NP_001156232.1 |
| cuticular protein 5 precursorb | 6.00E-40 | 97% | NP_001156154.1 |
| chemosensory protein-like precursorb | 1.00E-58 | 94% | NP_001119652.1 |
| coiled-coil-helix-coiled-coil-helix domain containingab | 3.00E-35 | 97% | NP_001155450.1 |
| titin-likeb | 0 | 86% | XP_008188078.1 |
| fibronectin type 3 domainb | 3.00E-05 | 69% | XP_008180552.1 |
| serine/arginine repetitive matrix protein 1ab | 2.00E-10 | 97% | XP_003242057.1 |
| Cu(I)-responsive transcriptional regulatorb | 3.00E-14 | 75% | XP_001948052.2 |
| jagunal homologb | 4.00E-44 | 97% | NP_001155727.1 |
| mitochondrial import inner membrane translocase subunit Tim22 isoform X1a | 9.00E-71 | 95% | XP_003245191.1 |
| EMI domain-containing proteina | 2.00E-60 | 85% | XP_001948474.2 |
aPrey proteins identified by bait BYDV-GPV CP.
bProteins identified by bait BYDV-GPV RTP.
abPrey proteins identified by both bait BYDV-GPV CP and RTP.