Figure 3.
Effect of sodium hydrosulfide (NaHS) on the immunoreactivity of P2X7 receptor in the cerebral cortex of rats with cerebral ischemia/reperfusion (I/R) injury.
(A–C) Sections were incubated with the relevant primary antibody and labeled with Cy3-conjugateed secondary antibody (red) and DAPI (blue) stain. (A) Sham group, (B) I/R group, (C) NaHS + I/R group. Arrows indicate cells immunoreactive for P2X7. (D) The number of P2X7-immunoreactive cells in the cerebral cortex of rats. **P < 0.01, vs. sham group; ##P < 0.01, vs. I/R group. The data are expressed as the mean ± SD (n = 10) and analyzed by one-way analysis of variance followed by the least significant difference test. Ten animals per group were used. Scale bars: 30 μm.