Figure 2.

Immunohistochemistry for p63 in the hippocampal CA1 region (left two columns) and CA2/3 regions (right two columns) of the adult and young gerbils in the sham- (A, B, a and b) and ischemia-operated (C–H and c–h) groups.

In the sham-operated young group, p63 immunoreactivity in the stratum pyramidale (SP, black asterisks) was significantly lower than that in the sham-operated adult group. In the ischemia-operated adult group, p63 immunoreactivity was significantly decreased in the SP (black asterisk) of the hippocampal CA1 region 4 days after ischemia/ reperfusion. However, at this time point, p63 immunoreactivity was distinctively increased in the SP (white asterisk) of the ischemia-operated young group, and p63 immunoreactive pyramidal neurons were significantly decreased 7 days after ischemia/reperfusion. Meanwhile, in the ischemia-operated adult group, p63 immunoreactivity was not changed in the SP of the hippocampal CA2/3 regions compared with that in the sham-operated adult group. In the ischemia-operated young group, p63 immunoreactivity was similar to that in the ischemia-operated adult group. SO: Stratum oriens; SR: stratum radiatum. Scale bars: 50 μm in A–H and 100 μm in a–h.