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. 2015 Feb 6;61(3):161–168. doi: 10.1262/jrd.2014-107

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©2015 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 6. — Trace of follicle growth of cultured secondary follicles for 1 week. (A) The graph indicates the result of tracing the follicle growth of follicles with a diameter of 110–120 μm. (B) The graph indicates the result of tracing the follicle growth of follicles with a diameter of 120–130 μm. (C) The graph indicates the result of tracing the follicle growth of follicles with a diameter 130 < μm. The black line indicates the follicles cultured in the normal medium (IGF1 (–)), and the dashed line indicates the follicles cultured in the medium containing IGF1 (50 ng/ml, IGF1 (+)).The numbers of samples per experiment were as follows: (A) IGF (–), 20; IGF1 (+), 29; (B) IGF1 (–), 24; IGF (+), 24; (C) IGF1 (–), 18; IGF (+), 15. Statistical significance is indicated by an asterisk (*P < 0.05, ** P < 0.01, *** P < 0.001). Values are presented as means ± standard deviation.