Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 6;61(3):179–184. doi: 10.1262/jrd.2014-137

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2015 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

PMC Copyright notice

Fig. 3. — Effect of Rnf20 knockdown on the progression of preimplantation development. The small interfering RNAs against Rnf20 (siRnf20 #1 and #2) or green fluorescent protein (siGFP) were injected into the cytoplasm of the embryos 2 h after insemination. Development to the 1-, 2- and 4-cell, morula and blastocyst (Blast.) stages was observed at 12, 28, 45, 70 and 96 h after insemination, respectively. Four independent experiments were performed, and the data were accumulated. A total of 79 embryos were examined in each experimental group. Black, white, grey and marble bars indicate no injection, siGFP, siRnf20 #1 and siRnf20 #2, respectively. Values indicated by asterisks are significantly lower than the control values (siGFP) (P < 0.01; χ²-test).