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. 2015 Feb 6;61(3):179–184. doi: 10.1262/jrd.2014-137

Fig. 4.

Fig. 4.

Effect of Rnf20 knockdown on the cell numbers in embryos at the blastocyst stage. The embryos were injected with small interfering RNAs against Rnf20 (siRnf20 #1 and #2) or green fluorescent protein (siGFP) 2 h after insemination and collected at the blastocyst stage 96 h after insemination. The numbers of cells in the whole embryos (A), in the inner cell mass (ICM; B) and in the trophectoderm (TE; C) were determined. For the determination of cell numbers in the ICM and TE, the nuclei of the embryos were stained differentially after immunosurgery. Four independent experiments were performed, and the data were accumulated. In each experimental group, more than 44 embryos were analyzed in total. Error bars indicate standard errors (SE). Values indicated by asterisks are significantly lower than those of the control embryos (siGFP) (based on the Student’s t-test; *P < 0.05; **P < 0.01).