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Effect of Rnf20 knockdown on the expression of histone H2A and H2B genes. The expression of histone H2A and H2B genes in the Rnf20 knockdown embryos was examined using RT-PCR. The embryos were injected with siRnf20 #1, siRnf20 #2 or siGFP 2 h after insemination and collected at the morula stage 70 h after insemination. Two independent experiments were performed, and similar results were obtained. A representative image is shown. Rabbit α-globin was used as an external control.
