TABLE I.
Sample | Enzymatic activity (unitsa) |
---|---|
Active f-PSA in assay buffer | 30.44 |
Inactive f-PSA in assay buffer | 0 |
Active f-PSA in HUVEC media | 29.35 |
Inactive f-PSA in HUVEC media (with serum)b | 0 |
Inactive f-PSA in HUVEC media (without serum)b | 0 |
PSA in seminal plasma | 35.95 |
Unit of enzymatic activity is defined as the amount of enzyme that cleaves 1 μM of substrate/min/ml.
Enzymatic activity was inhibited with 50 μM Zinc2+.