Figure 4.

Delivery of Lv5 and binding of Lv5 to Abl kinase in K562 cells. A) Construct of Lv5 containing HA4 (PDB ID: 3K2M), GS-rich linker, and 7c12 (PDB ID: 3T04). B) Left: western blot of total cell lysate from K562 cells treated for 24 h with 50 nm Lv5 or Lv5mut in the presence of 20 nm PA or PA[F427H]. Right: the lysate was then subjected to co-immunoprecipitation (co-IP) with anti-Abl agarose beads. Cells treated with Lv5mut/PA or Lv5/PA[F427H] served as negative controls for co-IP.