Figure 6.

H19 expression is associated with ER⁺ breast tumors. (A) Expression of the H19 gene was quantified in 39 ER⁺ and 31 ER⁻ primary breast tumors using qPCR. On average, the ER⁺ breast tumors showed higher expression of H19 (mean±s.d.=17.65±31.04) as compared with the ER⁻ tumors (8.11±9.4) as per the Mann–Whitney test (P=0.0030). (B) Based on a ligand binding assay, the expression of ERα was found to range from 0 to 302 fmol/mg protein. Pearson correlation revealed a positive correlation between ERα and H19 expression in the breast tumors (r=0.731).