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. 2015 Jul 11;46(1):80. doi: 10.1186/s13567-015-0212-1

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© Takanashi et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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rbCyPA possesses chemotactic activity. A Granulocytes were purified by centrifugation with Lympholyte-H and the purification was confirmed by staining with anti-granulocyte antibody. B Chemotactic analysis with several different concentration of rbCyPA was performed. Granulocytes migrating toward rbCyPA are shown. 1: 0 ng/mL, 2: 30 ng/mL, 3: 50 ng/mL, 4: 100 ng/mL, 5: 300 ng/mL, 6: 500 ng/mL of rbCyPA. C Chemotactic activity was evaluated by counting of granulocytes that migrated toward rbCyPA. Three separate experiments were performed and one representative result is shown. Values with different letters are statistically different (p < 0.01).