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. 2015 Jul 10;9(7):e0003925. doi: 10.1371/journal.pntd.0003925

Fig 7. S. japonicum MEG and VAL proteins are localized to the posterior esophageal gland.

Fig 7

Localization of target proteins on permeabilized whole worms (green, A, B and E to F), and cryosections (red, C and D) by immunocytochemistry, showing that all seven proteins, (A) MEG-4.1, (B) MEG-4.2, (C) MEG-8.2, (D) VAL-7, (E) MEG-11, (F) MEG-14 and (G) MEG-9 were solely expressed in the esophageal gland (on C & D, nuclei are blue and muscle is orange). Only the cytoplasm of the gland cell bodies (EG) was strongly positive, whilst the individual nuclei (N in Fig 7F) appeared as dark unstained spheres. The association of at least three proteins (MEG-4.2, MEG-8.2 and MEG-9) with host leucocytes in the lumen (starred, B and G) and the oral cavity (starred, G), or by a coating on both anterior and posterior esophageal linings (arrowed, C), confirmed that they were secreted. Scale bars: 50 μm (A, C, D), 20 μm (B, E, F, G).