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. 2015 Jul 10;10(7):e0132571. doi: 10.1371/journal.pone.0132571

Fig 3. F. tularensis infecting subsets of B cells in vitro.

Fig 3

Subsets of B cells were infected for 3 h with unopsonized F. tularensis LVS/GFP (GFP), F. tularensis LVS/GFP opsonized with fresh un-inactivated serum (GFP+C) from naïve mice, and bacteria opsonized with heat-inactivated immune sera (GFP+Ab). The proportions of infected CD19+ cells from all measured cells and of infected B-1a, B-1b, and B-2 cells from CD19+ cells were measured by flow cytometry. Error bars indicate SD around the means of samples processed in triplicate. Two-tailed t-test was used to test for significant differences between GFP and GFP+C and GFP+Ab (*** P < 0.001, ** P < 0.01, * P < 0.05). Results shown from one experiment are representative of three independent experiments.