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. 2015 Jul 10;10(7):e0132757. doi: 10.1371/journal.pone.0132757

Fig 2. IRF6 is required for Notch induced cell proliferation.

Fig 2

(A) Relative mRNA expression levels of Notch target gene HEY2 and of IRF6 in MCF10A cells infected with (i) two control viruses, (ii) control and IRF6 shRNA (shIRF6) expressing viruses, (iii) NICD and control shRNA expressing viruses or (iv) NICD and shIRF6 expressing viruses. (B) Representative dot plots of BrdU FACS analysis. R1 population (upper panel) were analyzed for BrdU-APC signal. R2 population (lower panel) shows events positive for BrdU-APC signal. (C) BrdU positive cell percentage in MCF10A cells, infected as indicated above, after 4 hours of BrdU incorporation. (D) MTT assay values indicating cell viability of MCF10A cells infected as indicated above. Values represent mean±S.D. of three independent experiments. (p values: *: < 0.05, **: <0.006).