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. 2015 Jul 10;10(7):e0132757. doi: 10.1371/journal.pone.0132757

Fig 4. Notch inhibition reduces IRF6 expression in MDA MB 231 cells.

Fig 4

Notch signaling was inhibited via overexpression of dominant negative mastermind (DNMM) (A and B) or silencing of Notch signaling mediator RBPjκ/CSL via shRNA (shCSL) (C and D). Relative mRNA expression levels of Notch target genes HEY1 and HEY2 and of IRF6 (A) and protein levels of IRF6 (B) 48 hours after infection with control (grey bars) or DNMM (black bars) expressing retrovirus (Left: representative western image, Right: Quantification of the protein bands). Relative mRNA expression levels of Notch target genes HEY1 and HEY2 and of IRF6 (C) and protein levels of IRF6 (D) 48 hours after infection with control (grey bars) or shCSL (black bars) expressing retrovirus (Left: representative western image, Right: Quantification of the protein bands). Values represent mean±S.D. of three independent experiments. (p values: *: <0.05, **: <0.004, ***: <0.0003).