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. 2015 Jul 10;10(7):e0131998. doi: 10.1371/journal.pone.0131998

Fig 3. The effect of IDH1 R132C on osteogenic properties of hMSCs.

Fig 3

A. The mRNA expression of RUNX2, OSX, ALPL and COL1A1 genes in hMSCs. RNAs were extracted from each hMSC and analyzed by qRT-PCR. Data are shown as a value relative to that of parental cells after normalizing to expression of the ACTB gene. B. Calcium (Ca) deposition after osteogenic induction in each hMSC. Data were normalized to the culture area. Results (A and B) are the mean ± SE from the results of three different donors. C. Calcified nodule formation after osteogenic induction. Each type of BM01 cell was cultured under osteogenic induction conditions and then stained with Alizarin Red S. **, p<0.01 by Dunnett`s multiple comparisons test compared to the parental cells. Par, EV, MT, and R132C were as described in the legend for Fig 1.