Table 2.
Ascidian species (classified at least to the genus level) found in the 32 surveyed harbors
| Order | Species | Origin | Acc. num. | # Harbors |
|---|---|---|---|---|
| Aplousobranchia | Clavelina oblonga | Introduced | KF309648 | 1 |
| Clavelina lepadiformis | Introduced | KF309563, -638 | 32 | |
| Clavelina sabbadini | Native | KF309535, -645 | 2 | |
| Diplosoma listerianum | Introduced | KF309531, -561, -581, -605, -616, -638, -639, -660, -664 | 30 | |
| Diplosoma spongiforme | Native | KF309624 | 2 | |
| Trididemnum cereum | Native | KF309632 | 3 | |
| Didemnum sp. 1 | – | KF309573 | 1 | |
| Didemnum sp. 2 | – | KF309622 | 1 | |
| Didemnum fulgens | Native | KF309576 | 1 | |
| Morchellium argus | Native | KF309620-21 | 1 | |
| Aplidium accarense | Introduced | KF309553, -558, -571, -574, -584–586, -597–599, -601, -646, -618, -625–27, -629–630, -640, -654–657, -663 | 23 | |
| Aplidium sp. 1 | – | – | 1 | |
| Aplidium sp. 2 | – | KF309633 | 1 | |
| Phlebobranchia | Ascidia virginea | Native | KF309647 | 1 |
| Ascidia sp. | – | – | 1 | |
| Ascidiella aspersa | Introduced | KF309533-34, -555, -559, -562, -568, -594, -606, -617, -631, -637, -653, -661 | 27 | |
| Ascidiella scabra | Cryptogenic | KF309529, -556, -560, -572, -650 | 5 | |
| Phallusia ingeria | Native | – | 1 | |
| Phallusia sp. | – | – | 1 | |
| Phallusia mammillata | Native | KF309607 | 1 | |
| Phallusia fumigata | Native | KF309548 | 1 | |
| Ciona intestinalis | Cryptogenic | KF309614, -532, -554, -570, -574, -578, -580, -587, -591, -593, -602–604, -613, -628, -651, -658, -662 | 28 | |
| Ciona sp. | – | KF309636 | 1 | |
| Stolidobranchia | Botrylloides leachii | Cryptogenic | KF309549, -551, -608–611, -641–642, -644 | 3 |
| Botryllus schlosseri | Cryptogenic | KF309536-47, -530, -564–567, -579, -592, -615, -659 | 30 | |
| Polyandrocarpa zorritensis | Introduced | KF309643 | 1 | |
| Distomus variolosus | Native | KF309623 | 2 | |
| Styela plicata | Introduced | – | 21 | |
| Styela canopus | Cryptogenic | KF309590 | 2 | |
| Polycarpa pomaria | Native | – | 2 | |
| Polycarpa sp. | – | KF309588 | 1 | |
| Molgula bleizi | Native | – | 2 | |
| Molgula occidentalis | Native | – | 1 | |
| Molgula sp. | – | – | 1 | |
| Microcosmus squamiger | Introduced | KF309550, -552, -595, -600, -612, -619, -634–635, -649, -652 | 24 | |
| Pyura dura | Native | KF309596 | 4 | |
| Pyura squamulosa | Native | – | 2 |
Species were further classified according to their origin: native, introduced, and cryptogenic (see Appendix 1). GenBank accession numbers of the COI sequences generated in this study and the number of harbors in which the species have been found are also indicated