Table 2. Adhesion of different A. hydrophila serotype O34 strains to HEp-2 cells.
Strain and main characteristics | Mean n°. of bacteria/ HEp-2 cell +/- SD | % Reduction in adhesion a |
---|---|---|
AH-3; wild type | 18.3 +/- 2.2 | |
AH-405; AH-3 rifampicin-resistant mutant | 17.9 +/- 2.0 | 2 b |
AH-3Δ wecP | 7.2 +/- 0.7 | 61 |
AH-3Δ wecP + pBAD33 | 6.9 +/- 1.0 | 62 |
AH-3Δ wecP + pBAD-WecPAh | 17.7 +/- 2.1 | 3 |
AH-3Δ wecP + pBAD33-WecAEc | 12.7 +/- 1.4 | 30 |
AH-3Δ wecP + pBAD-WecA-WzyEEc | 18.0 +/- 2.5 | 1 |
AH-3Δ wecP + pBAD-WzyEEc | 6.7 +/- 0.5 | 63 |
a The level of adhesion of strain AH-3 was used as 100% value.
b Student’s t test, P, 0.001.
The strains carrying pBAD plasmids were grown under inducing conditions (+ arabinose)