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. 2015 Jun 27;71(Pt 7):806–814. doi: 10.1107/S2053230X15008626

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© Pusey et al. 2015

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Fluorescent bright spots as crystallization lead conditions. The pairs of images to the left are the transmission (white light) and fluorescent images of the initial crystallization screen. The images to the left are the transmission and fluorescent images of the optimized outcomes. (a) HiIPPase, lead Hampton Research Crystal Screen HT (HRHT) condition E1, optimization with 90% E1 + 0.1 M 1-ethyl-3-methylimidazolium chloride. (b) Tt169, lead HRHT condition D6, optimization using Crystal Former HT plates (Microlytic/Anatrace). The capillary width is 100 µm and the imaged volume is ∼15 nl. (c) Tt97, lead HRHT condition C2; the capillary in (c) has an internal diameter of 200 µm.