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. 2015 Jun 27;71(Pt 7):831–837. doi: 10.1107/S2053230X1500967X

Table 1. Formulation of MORPHEUS II.

A mix of precipitants includes a high-molecular-weight PEG and a cryoprotectant (small polyol). Two precipitant mixes also include NDSBs. The formulations of the eight additive mixes can be found in Table 3 ▸. The formulations of the three buffer systems can be found in Supplementary Table S2. The roman numeral II was used to distinguish the new mixes from similarly named ones in the original MORPHEUS screen. AMPD, 2-amino-2-methyl-1,3-propanediol; BES, N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid; bis-tris, bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane; GlyGly, glycylglycine; HEPES, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonate; MES, 2-(N-morpholino)ethanesulfonic acid; MOPSO, 3-morpholino-2-hydroxypropanesulfonic acid; TEA, triethanolamine.

Well Mix of precipitants Mix of additives Buffer system
A1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.03M of each LiNaK 0.1M MOPSO/bis-tris pH 6.5
A2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.03M of each LiNaK 0.1M MOPSO/bis-tris pH 6.5
A3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.03M of each LiNaK 0.1M MOPSO/bis-tris pH 6.5
A4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.03M of each LiNaK 0.1M MOPSO/bis-tris pH 6.5
A5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.03M of each LiNaK 0.1M BES/TEA pH 7.5
A6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.03M of each LiNaK 0.1M BES/TEA pH 7.5
A7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.03M of each LiNaK 0.1M BES/TEA pH 7.5
A8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.03M of each LiNaK 0.1M BES/TEA pH 7.5
A9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.03M of each LiNaK 0.1M GlyGly/AMPD pH 8.5
A10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.03M of each LiNaK 0.1M GlyGly/AMPD pH 8.5
A11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.03M of each LiNaK 0.1M GlyGly/AMPD pH 8.5
A12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.03M of each LiNaK 0.1M GlyGly/AMPD pH 8.5
B1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Divalent cation II 0.1M MOPSO/bis-tris pH 6.5
B2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Divalent cation II 0.1M MOPSO/bis-tris pH 6.5
B3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Divalent cation II 0.1M MOPSO/bis-tris pH 6.5
B4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Divalent cation II 0.1M MOPSO/bis-tris pH 6.5
B5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Divalent cation II  
B6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Divalent cation II  
B7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Divalent cation II  
B8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Divalent cation II  
B9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Divalent cation II 0.1M GlyGly/AMPD pH 8.5
B10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Divalent cation II 0.1M GlyGly/AMPD pH 8.5
B11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Divalent cation II 0.1M GlyGly/AMPD pH 8.5
B12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Divalent cation II 0.1M GlyGly/AMPD pH 8.5
C1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 1mM of each Alkali 0.1M MOPSO/bis-tris pH 6.5
C2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 1mM of each Alkali 0.1M MOPSO/bis-tris pH 6.5
C3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 1mM of each Alkali 0.1M MOPSO/bis-tris pH 6.5
C4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 1mM of each Alkali 0.1M MOPSO/bis-tris pH 6.5
C5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 1mM of each Alkali 0.1M BES/TEA pH 7.5
C6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 1mM of each Alkali 0.1M BES/TEA pH 7.5
C7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 1mM of each Alkali 0.1M BES/TEA pH 7.5
C8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 1mM of each Alkali 0.1M BES/TEA pH 7.5
C9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 1mM of each Alkali 0.1M GlyGly/AMPD pH 8.5
C10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 1mM of each Alkali 0.1M GlyGly/AMPD pH 8.5
C11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 1mM of each Alkali 0.1M GlyGly/AMPD pH 8.5
C12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 1mM of each Alkali 0.1M GlyGly/AMPD pH 8.5
D1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Oxometalate 0.1M MOPSO/bis-tris pH 6.5
D2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Oxometalate 0.1M MOPSO/bis-tris pH 6.5
D3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Oxometalate 0.1M MOPSO/bis-tris pH 6.5
D4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Oxometalate 0.1M MOPSO/bis-tris pH 6.5
D5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Oxometalate 0.1M BES/TEA pH 7.5
D6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Oxometalate 0.1M BES/TEA pH 7.5
D7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Oxometalate 0.1M BES/TEA pH 7.5
D8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Oxometalate 0.1M BES/TEA pH 7.5
D9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Oxometalate 0.1M GlyGly/AMPD pH 8.5
D10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Oxometalate 0.1M GlyGly/AMPD pH 8.5
D11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Oxometalate 0.1M GlyGly/AMPD pH 8.5
D12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Oxometalate 0.1M GlyGly/AMPD pH 8.5
E1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Lanthanide 0.1M MOPSO/bis-tris pH 6.5
E2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Lanthanide 0.1M MOPSO/bis-tris pH 6.5
E3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Lanthanide 0.1M MOPSO/bis-tris pH 6.5
E4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Lanthanide 0.1M MOPSO/bis-tris pH 6.5
E5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Lanthanide 0.1M BES/TEA pH 7.5
E6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Lanthanide 0.1M BES/TEA pH 7.5
E7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Lanthanide 0.1M BES/TEA pH 7.5
E8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Lanthanide 0.1M BES/TEA pH 7.5
E9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.5mM of each Lanthanide 0.1M GlyGly/AMPD pH 8.5
E10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.5mM of each Lanthanide 0.1M GlyGly/AMPD pH 8.5
E11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.5mM of each Lanthanide 0.1M GlyGly/AMPD pH 8.5
E12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.5mM of each Lanthanide 0.1M GlyGly/AMPD pH 8.5
F1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Monosaccharide II 0.1M MOPSO/bis-tris pH 6.5
F2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Monosaccharide II 0.1M MOPSO/bis-tris pH 6.5
F3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Monosaccharide II 0.1M MOPSO/bis-tris pH 6.5
F4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Monosaccharide II 0.1M MOPSO/bis-tris pH 6.5
F5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Monosaccharide II 0.1M BES/TEA pH 7.5
F6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Monosaccharide II 0.1M BES/TEA pH 7.5
F7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Monosaccharide II 0.1M BES/TEA pH 7.5
F8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Monosaccharide II 0.1M BES/TEA pH 7.5
F9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Monosaccharide II 0.1M GlyGly/AMPD pH 8.5
F10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Monosaccharide II 0.1M GlyGly/AMPD pH 8.5
F11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Monosaccharide II 0.1M GlyGly/AMPD pH 8.5
F12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Monosaccharide II 0.1M GlyGly/AMPD pH 8.5
G1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Amino-acid II 0.1M MOPSO/bis-tris pH 6.5
G2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Amino-acid II 0.1M MOPSO/bis-tris pH 6.5
G3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Amino-acid II 0.1M MOPSO/bis-tris pH 6.5
G4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Amino-acid II 0.1M MOPSO/bis-tris pH 6.5
G5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Amino-acid II 0.1M BES/TEA pH 7.5
G6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Amino-acid II 0.1M BES/TEA pH 7.5
G7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Amino-acid II 0.1M BES/TEA pH 7.5
G8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Amino-acid II 0.1M BES/TEA pH 7.5
G9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.02M of each Amino-acid II 0.1M GlyGly/AMPD pH 8.5
G10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02M of each Amino-acid II 0.1M GlyGly/AMPD pH 8.5
G11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.02M of each Amino-acid II 0.1M GlyGly/AMPD pH 8.5
G12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.02M of each Amino-acid II 0.1M GlyGly/AMPD pH 8.5
H1 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.01M of each Polyamine 0.1M MOPSO/bis-tris pH 6.5
H2 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.01M of each Polyamine 0.1M MOPSO/bis-tris pH 6.5
H3 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.01M of each Polyamine 0.1M MOPSO/bis-tris pH 6.5
H4 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.01M of each Polyamine 0.1M MOPSO/bis-tris pH 6.5
H5 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.01M of each Polyamine 0.1M BES/TEA pH 7.5
H6 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.01M of each Polyamine 0.1M BES/TEA pH 7.5
H7 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.01M of each Polyamine 0.1M BES/TEA pH 7.5
H8 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.01M of each Polyamine 0.1M BES/TEA pH 7.5
H9 15%(w/v) PEG 3K, 20%(v/v) 1,2,4-butanetriol, 1%(w/v) NDSB 256 0.01M of each Polyamine 0.1M GlyGly/AMPD pH 8.5
H10 12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.01M of each Polyamine 0.1M GlyGly/AMPD pH 8.5
H11 10%(w/v) PEG 8K, 20%(v/v) 1,5-pentanediol 0.01M of each Polyamine 0.1M GlyGly/AMPD pH 8.5
H12 5%(w/v) PEG 20K, 25%(w/v) 1,1,1-tris(hydroxymethyl)propane, 1%(w/v) NDSB 195 0.01M of each Polyamine 0.1M GlyGly/AMPD pH 8.5