Table 1. Macromolecule-production information.

Source organism	T. maritima (Gene ID 897867)
DNA source	Synthetic DNA (codon-optimized)
Forward primer†	CGGGATCCATGGGCAGCAGCCATCATCA
Reverse primer‡	CCGCTCGAGTTAGCCCAGACTATCCAGCA
Cloning vector	pET-28a(+)
Expression vector	pET-28a(+)
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced§	MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMLYKELGRTGEEIPALGLGTWGIGGFETPDYSRDEEMVELLKTAIKMGYTHIDTAEYYGGGHTEELIGKAIKDFRREDLFIVSKVWPTHLRRDDLLRSLENTLKRLDTDYVDLYLIHWPNPEIPLEETLSAMAEGVRQGLIRYIGVSNFDRRLLEEAISKSQEPIVCDQVKYNIEDRDPERDGLLEFCQKNGVTLVAYSPLRRTLLSEKTKRTLEEIAKNHGATIYQIMLAWLLAKPNVVAIPKAGRVEHLRENLKATEIKLSEEEMKLLDSLGQ

^†The underlined sequence is the BamHI site.

^‡The underlined sequence is the XhoI site.

^§The underlined sequence indicates the vector-derived amino acids of the recombinant Tm1743 enzyme, including the thrombin cleavage site (LVPRGS).