Table 2. Initial crystallization conditions and optimization results for the Tm1743NADP⁺EOPB sample.

Tm1743 (20mgml¹) was incubated with NADP⁺ and EOPB in a 1:1.5:1.5 molar ratio for 2h at 277K before crystallization. Crystallization trials were performed in 24-well plates using the hanging-drop vapour-diffusion method.

Temperature (K)	Condition	Initial condition	Crystal shape	Optimized condition	Diffraction
277	Crystal Screen No. 24	0.2M calcium chloride dehydrate, 0.1M sodium acetate trihydrate pH 4.6, 20%(v/v) 2-propanol	Cubic	0.2M calcium chloride dehydrate, 0.1M sodium acetate trihydrate pH 4.6, 25%(v/v) 2-propanol	No diffraction
289	PEGRx 2 No. 30	0.2M magnesium formate dehydrate, 0.1M sodium acetate trihydrate pH 4.0, 18%(v/v) PEG MME 5000	Irregular octahedral	Irreproducible	No diffraction
289	PEGRx 2 No. 45	5%(v/v) 2-propanol, 0.1M citric acid pH 3.5, 6%(w/v) PEG 20000	Irregular octahedral	Irreproducible	No diffraction
298	Crystal Screen 2 No. 34	0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.0M sodium acetate trihydrate	Regular dodecahedral	0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.2M sodium acetate trihydrate; enzyme concentration 15mgml1	Diffracted to 2 resolution (Fig. 1 ▸ a)