| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
24-well crystallization plates |
24-well crystallization plates |
| Temperature (K) |
298 |
298 |
| Enzyme concentration (mgml1) |
15 |
12 |
| Buffer composition of enzyme |
25mM TrisHCl pH 8.5 |
25mM TrisHCl pH 8.5 |
| Composition of reservoir solution |
0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.2M sodium acetate trihydrate |
0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.3M sodium acetate trihydrate |
| Volume and ratio of drop |
3l, 1:1 |
3l, 1:1 |
| Volume of reservoir (l) |
200 |
200 |
| Crystal image |
Fig. 1 ▸(a) |
Fig. 1 ▸(b) |