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. 2015 Jun 27;71(Pt 7):847–855. doi: 10.1107/S2053230X15009735

Table 3. Crystallization of Tm1743 in complex with NADP+ .

Sample Tm1743NADP+EOPB Tm1743NADP+
Method Hanging-drop vapour diffusion Hanging-drop vapour diffusion
Plate type 24-well crystallization plates 24-well crystallization plates
Temperature (K) 298 298
Enzyme concentration (mgml1) 15 12
Buffer composition of enzyme 25mM TrisHCl pH 8.5 25mM TrisHCl pH 8.5
Composition of reservoir solution 0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.2M sodium acetate trihydrate 0.05M cadmium sulfate hydrate, 0.1M HEPES pH 7.5, 1.3M sodium acetate trihydrate
Volume and ratio of drop 3l, 1:1 3l, 1:1
Volume of reservoir (l) 200 200
Crystal image Fig. 1(a) Fig. 1(b)