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. 2015 Jun 27;71(Pt 7):870–875. doi: 10.1107/S2053230X15005956

Table 1. Macromolecule-production information for the AtBAG5CaM complex.

  AtBAG5 CaM
Source organism A. thaliana Rattus norvegicus
DNA source A. thaliana leaf cDNA R. norvegicus cDNA
Forward primer 5-CCGAATTCAACGCCACCGCCGCAGCA-3 5-CCGGATCCATGGCTGACCAACTGACTGAAGAGCAG-3
Reverse primer 5-CCCTCGAGCTAGTCTTTAGTCTCCGAGATTGAATCGAG-3 5-CCCTCGAGCTATCATTTTGCAGTCATCATTTGTACAAAC-3
Cloning vector pEasy Blunt simple cloning vector pEasy Blunt simple cloning vector
Expression vector Modified pET-32a (contains only the N-terminal His6 tag and a PreScission protease cleavage site) pET-33b
Expression host E. coli BL21(DE3) CodonPlus E. coli BL21(DE3) CodonPlus
Complete amino-acid sequence of the construct produced GPEFNATAAAARIQSGYRSYRIRNLYKKISSINREANRVQSIIQRQETVDAIRSDEKERLRMNETLMALLLKLDSVPGLDPTIREARRKVSRKIVGMQEILDSISETKD GSMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK