| Source organism |
A. thaliana
|
Rattus norvegicus
|
| DNA source |
A. thaliana leaf cDNA |
R. norvegicus cDNA |
| Forward primer |
5-CCGAATTCAACGCCACCGCCGCAGCA-3 |
5-CCGGATCCATGGCTGACCAACTGACTGAAGAGCAG-3 |
| Reverse primer |
5-CCCTCGAGCTAGTCTTTAGTCTCCGAGATTGAATCGAG-3 |
5-CCCTCGAGCTATCATTTTGCAGTCATCATTTGTACAAAC-3 |
| Cloning vector |
pEasy Blunt simple cloning vector |
pEasy Blunt simple cloning vector |
| Expression vector |
Modified pET-32a (contains only the N-terminal His6 tag and a PreScission protease cleavage site) |
pET-33b |
| Expression host |
E. coli BL21(DE3) CodonPlus |
E. coli BL21(DE3) CodonPlus |
| Complete amino-acid sequence of the construct produced |
GPEFNATAAAARIQSGYRSYRIRNLYKKISSINREANRVQSIIQRQETVDAIRSDEKERLRMNETLMALLLKLDSVPGLDPTIREARRKVSRKIVGMQEILDSISETKD |
GSMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |