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| Method | Sitting-drop vapour diffusion |
| Plate type | 48-well sitting-drop plates |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | 20mM TrisHCl pH 7.5, 500mM NaCl, 1mM DTT, 5mM EDTA |
| Composition of reservoir solution | 2.5M ammonium sulfate, 0.1M TrisHCl pH 8.5 |
| Volume (l) and ratio of drop | 2l in total, 1:1 |
| Volume of reservoir (l) | 100 |
