Figure 8.

(a) Fluorescence anisotropy assays using ctMex67^ΔN–Mtr2. Data were fitted to the standard quadratic binding equation to obtain K _d values. All four RNA oligonucleotides tested were bound, albeit with different affinities. Similar to scMex67–Mtr2, ctMex67^ΔN–Mtr2 bound polyA₁₅ and polyG₁₅ more tightly than polyU₁₅ and polyG₁₅. (b) Deletion of either the RRM or NTF2L–Mtr2 domains reduced the affinity of ctMex67 by >15-fold. On the other hand, deletion of the UBA domain from ctMex67^ΔN–Mtr2 did not reduce the affinity towards polyA₁₅ RNA significantly. Curves reproduced from (a) are shown as dotted lines without data points.