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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plate |
| Temperature (K) | 289 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | 25mM bis-tris pH 6.0, 100mM NaCl |
| Composition of reservoir solution | 100mM bis-tris pH 6.0, 200mM ammonium sulfate, 17%(w/v) PEG 3350 |
| Volume and ratio of drop | 1l:1l:0.2l protein:reservoir:3.3% DDM |
| Volume of reservoir (l) | 200 |
