Figure 3.

The L-CDR3 loop is stabilized in the crystal by interaction with the neighbouring molecule. (a) Two adjacent symmetry-related molecules, forming the homodimer by interacting with each other, are shown as green and cyan ribbons. The L-CDR3 loop is depicted in sticks. An F _o − F _c OMIT map (red mesh, omitting residues 171–188) shows the electron density of the L-CDR3 loop between two molecules contoured at 3σ. (b) The detailed electron density (red mesh) from the F _o − F _c OMIT map (2σ) shows the residues of the L-CDR3 loop (modelled as blue sticks). The residues from GFP are modelled as green sticks and cartoons. (c) The L-CDR3 loop residues Gln₁, His, Pro and Lys interact with a neighbouring GFP barrel. The neighbouring GFP barrel is shown as a blue ribbon and the L-CDR3 loop is shown as a green ribbon. The residues involved in the interaction are shown in sticks. Hydrophobic interactions are shown as yellow dashed lines and two hydrogen bonds are shown as red dashed lines.