Table 4.
Physicochemical properties of the PTX/DM-β-CD inclusion complex-loaded CS nanoparticles
| PTX/DM-β-CD (mol/mol) | Weight ratio of PTX to CS | EE (%) | LE (%) | Particle size (nm) | Zeta potential (mV) | PI |
|---|---|---|---|---|---|---|
| 1/0 | 0 | ND | ND | 238.5±28.1 | 41.1±1.2 | 0.21 |
| 1/0 | 0.7 | 9.13±2.23 | 0.13±0.01 | 258.1±16.7 | 13.1±5.2 | 0.22 |
| 1/0 | 1 | 8.77±1.11 | 0.15±0.01 | 250.6±16.4 | 18.5±3.2 | 0.20 |
| 1/50 | 0.5 | 65.33±3.12 | 2.35±0.78 | 323.9±18.0 | 23.3±4.5 | 0.25 |
| 1/50 | 0.7 | 82.74±6.59 | 3.97±0.45 | 355.6±21.9 | 22.4±4.3 | 0.24 |
| 1/50 | 1 | 74.32±8.56 | 3.04±0.39 | 407.8±22.9 | 15.9±2.7 | 0.26 |
Notes: Given values are means ± SD (n=3). Five milliliters of a 1 mg/mL chitosan solution were adjusted to a pH of 4.5. The calculated amount of 2 mg/mL PTX in ethanol was added to the chitosan solution at room temperature. Then, 1 mg/mL TPP (1 mL) was added dropwise to the chitosan solution with magnetic stirring at 800 rpm for 30 minutes.
Abbreviations: CS, chitosan; DM-β-CD, (2,6-di-O-methyl)-β-cyclodextrin; EE, entrapment efficiency; LE, loading efficiency; ND, no data; PI, polydispersity index; PTX, paclitaxel; SD, standard deviation; TPP, tripolyphosphate.