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. 2015 Jul 10;10(7):e0132431. doi: 10.1371/journal.pone.0132431

Fig 6. Detection of two shunt products in the mutant strain.

Fig 6

A: HPLC spectra of metabolites by the wild type strain (WT/pIJ8600), mutant strain (MS/pIJ8600) and control strain (CH999/pRM5), indicated as UV absorption at 434 nm. Control: CH999/pRM5 was a recombinant strain of S. coeliololar able to accumulate two shunt products DMAC 5 (Mr: 298, 16.7 min) and aloesaponarin II 6 (Mr. 254, 22.6 min), due to the presence of 6 genes on pRM5, encoding KS, CLF, ACP, KR, ARO and CYC (Fig 1B) from the earlier stages of ACT 1 pathway. These 6 genes control the formation of the bicyclic intermediate 4. Then the bicyclic intermediate was spontaneously converted into two shunt products in CH999/pRM5 (Fig 1B). B: Mass spectra of peaks for authentic DMAC 5 and aloesaponarin II 6 by CH999/pRM5 and for the compounds at 16.9 min and 22.6 min by the med-ORF12- deficient mutant strain.