Table 1. Primers used in this study.

Name	a Sequences (5’-3’)	PCR products
med12-L1	5’-CCCAAGCTTGGTATCGGCACCTCTTC (HindIII)	1.5 kb DNA located in the upstream of med-ORF12
med12-L1	5’-GCTCTAGA CATCGTTTTCG TTCTCCCG (XbaI)
med12-R2	5’-GCTCTAGA TGAGGGCGCCGTGCGGTC (XbaI)	1.3 kb DNA located in the downstream of med-ORF12
med12-R2	5’-GGAATTCAAGCTTGCGGACCAGAACCAG (EcoRI-HindIII)
med12-qc1	5’-CCGTCCTCCGTGGTGATCTCGAAG	1.7 kb/0.5 kb fragment (wild type/mutant) flanking upstream and downstream of med-ORF12
med12-qc2	5’-GTTGACGACGAACTCGGACGCGGC
med12-A	5’-GGAATTCCATATGAGCGGAACCGGCCGGCC (EcoRI- NdeI)	0.975 kb full-length med-ORF12
med12-B	5’-CGGGATCC TCACGACGCGCTCCC GGGCT (BamHI)

Note:

^a: Sequences underlined in the primers were recognition sites for restriction enzymes indicated in parentheses. Start/stop codons (ATG/TGA) were shown in bold.