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. 2015 Jul 10;10(7):e0132621. doi: 10.1371/journal.pone.0132621

Fig 5. qRT-PCR validation of some miRNAs and their targets identified by Solexa sequencing.

Fig 5

qRT-PCR reactions were used to verify the sequencing and the target predictions. (A) The result of the qRT-PCR validation of some miRNAs were identified through sequencing. The expression was represented as the ratios of the expression under SD treatment to that under LD treatment, and the 5SrRNA was used as a control. (B) The results of the qRT-PCR validation of the target predictions for miR159e-3p. The expression was presented as the ratio of expression under SD and LD treatment, and the 18SrRNA acted as a beta-actin (C) The result of the qRT-PCR validation of the target predictions for miR156a. (D) The result of qRT-PCR validation of the target predictions for miR160. (E) The results of qRT-PCR validation of the target predictions for miR395 and miR408. (F) The result of qRT-PCR validation of some miRNAs identified by sequencing.