Table 1. The novel soybean miRNAs verified using qRT-PCR.

miRNA ID	Sequence (5’-3’)	Length (nt)	Start/end precursor	Length of precursors (nt)	Arm	MFE (kcal/mol)
Soybean-miR-4	AAUGAGAACUUUGAAGGCCGAA	22	9955786–9955874	88	3p	-18.10
Soybean-miR-35	GGAAUGGGCUGAUUGGGAA	19	7783821–7783912	92	5p	-45.70
Soybean-miR-36	GGAGUGAAAUAGAACAUGAAA	21	13036509–13036600	91	5p	-20.00
Soybean-miR-57	UAUGGGGGGAUUGGGAAGGAA	21	35360317–35360403	86	5p	-40.30
Soybean-miR-60	UCUUCCCUACACCUCCCAUACC	22	35360314–35360403	89	3p	-43.50
Soybean-miR-64	UGAGAACUUUGAAGGCCGAA	20	9955786–9955874	88	3p	-18.10
Soybean-miR-77	UUCUGAACCAGACUUUGAUGUC	22	20678566–20678664	98	5p	-25.50