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. 2015 Jul 10;10(7):e0130514. doi: 10.1371/journal.pone.0130514

Table 1. Ingredients and chemical composition of experimental diets.

Ingredients a (g/kg as fed basis)
Diet MD CTRL
Wheat 446 446
Peas 231 231
Soybean meal 161 161
Sugarbeet molasses 20 20
Corn starch 88 86
Sunflower oil 10 10
l-Lys HCl 4.35 4.35
l-Thr 2.48 2.48
l-Trp 0.89 0.89
dl-Met 0 2.6
l-Ile 0.78 0.78
l-Val 1.48 1.48
l-Leu 1.18 1.18
Salt 4.5 4.5
Calcium carbonate 11 11
Dicalcium phosphate 12 12
Vitamin/mineral premix 5 5
Analyzed composition b (%)
Crude protein 18.0 18.2
Starch 39.2 38.8
Ash 5.4 5.1
Fat 2.4 2.4
Gross energy, (MJ/kg) 15.7 15.7
Net energyd, (MJ/kg) 9.95 9.96
Lys 1.31 1.28
Met 0.27 0.49
Cys 0.28 0.27
Thr 0.76 0.75
Trp 0.29 0.29
Val 0.99 0.97
Ile 0.82 0.79
Leu 1.31 1.29
Phe 0.82 0.79
Tyr 0.63 0.61
His 0.50 0.42
Arg 1.08 1.08
Ser 0.77 0.76
Gly 0.80 0.71
Ala 0.66 0.67
Asp 1.76 1.68
Glu 3.30 3.28
Pro 0.93 0.91
Calculated composition c , d (%)
SID Lys 1.17 1.15
SID Met 0.24 0.47
SID TSAA 0.47 0.69
SID Met:Lys 20 41
SID TSAA:Lys 40 60

Abbreviation used: TSAA, total sulfur amino acid.

a MD, a diet deficient in methionine; CTRL, a control diet.

b Adjusted for 87.3% of dry matter.

c Values were calculated from Sauvant et al. [19].

d SID, standardized ileal digestible amino acids. The values were obtained from the calculated ileal digestibility values of the ingredients combined with the measured amino acid contents.