Table 2. Lipid content and lipogenic enzyme activities.
| Diet a | MD | CTRL | RSD | P value |
|---|---|---|---|---|
| Number of pigs | 6 | 6 | ||
| PRAT | ||||
| Relative tissue weight | 0.37 | 0.29 | 0.07 | 0.08 |
| Lipid content b | 65.9 | 62.9 | 5.10 | 0.37 |
| Fatty acid synthase b | 93.6 | 78.0 | 16.1 | 0.15 |
| Malic enzyme b | 660 | 418 | 136 | 0.02 |
| G6PDH b | 341 | 253 | 48.0 | 0.02 |
| SCAT | ||||
| Lipid content | 66.2 | 59.5 | 4.60 | 0.04 |
| Fatty acid synthase | 40.4 | 26.2 | 11.0 | 0.06 |
| Malic enzyme | 423 | 224 | 91.0 | <0.01 |
| G6PDH | 233 | 145 | 31.0 | <0.001 |
| LD | ||||
| Lipid content | 2.80 | 2.50 | 0.30 | 0.08 |
| Fatty acid synthase | 0.30 | 0.20 | 0.04 | 0.64 |
| Malic enzyme | 9.10 | 5.50 | 2.70 | 0.06 |
| G6PDH | 4.50 | 3.60 | 1.00 | 0.15 |
| Liver | ||||
| Lipid content | 1.90 | 1.90 | 0.30 | 0.98 |
| Fatty acid synthase | 12.4 | 14.6 | 2.80 | 0.23 |
| Malic enzyme | 6.20 | 5.50 | 2.20 | 0.58 |
| G6PDH | 56.0 | 41.1 | 8.50 | 0.02 |
Abbreviation used: G6PDH, glucose-6-phosphate dehydrogenase; LD, longissimus dorsi muscle; PRAT, perirenal fat; SCAT dorsal subcutaneous adipose tissue.
a Pigs (42 d of age) were fed either a methionine-deficient (MD) diet or a control (CTRL) diet providing an adequate level of methionine for 10 days. Values are least square means together with residual standard deviation (RSD).
b Lipid content was expressed in g per 100 g of tissue fresh weight. Activities were expressed in nmoles of NADPH produced (malic enzyme, G6PDH) or consumed (fatty acid synthase) per min and per mg cytosolic proteins.