Fig 3. Circulating Reticulocytes in control and cadmium treated mice.

Mice were given cadmium chloride dissolved in drinking water (50 ppm and 1000 ppm). At different time points after cadmium exposure, mouse erythrocytes were stained ex vivo with anti-mouse CD71-PE and the proportion of circulating reticulocytes were determined by flow cytometry. Reticulocyte count in control ranged between 3.09 to 4.73% (mean = 3.77 ± 0.24). Mean reticulocyte counts in control group of mice at each time point was taken as 100 and the proportion of reticulocytes in the exposed groups were estimated in relative terms. The relative proportions of reticulocytes in 50 ppm and 1000 ppm groups of mice have been shown. Each point on the graph represents mean ± SEM of observations on 7–10 mice. *p<0.05 and **p<0.01 for comparison of the groups. Statistical analysis was done using Student t-test.